Genome folding is not static but emerges from dynamic processes that control transcription, replication, recombination, and repair. DNA loop extrusion by cohesin is central to genome organization, yet it remains unclear how cells can tune extrusion …
Cohesin drives genome organization via loop extrusion, orchestrated by the dynamic exchange of multiple essential accessory proteins. Although these regulators bind the core cohesin complex only transiently, their disruption can dramatically alter …
Enhancers have been proposed to act as privileged loading sites for cohesin, raising the idea that they actively fold the genome to engage distal target promoters for transcription. Supporting this idea, NIPBL/MAU2, which is required for cohesin …
Three-dimensional genome organization constrains the regulatory interactions that govern vital cellular processes. Chromatin loops are key features of genome folding, yet it is unclear how genetic and epigenetic variation influences differential loop …
Central to genome function, enhancers are non-coding sequences that can control transcription from promoters hundreds of kilobases away. Yet the physical basis of this long-range communication remains unclear. A prevalent view is that enhancers …
Enhancers are critical genetic elements controlling transcription from promoters, yet how they convey regulatory information across large genomic distances remains unclear. Here, we engineer pluripotent stem cells in which cohesin loop extrusion can …
The spatial folding of the genome shapes gene regulation by controlling which loci interact, yet inferring the mechanisms behind these 3D structures from contact maps remains difficult. Cohesin-mediated loop extrusion is a key organizer of domains …